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ACM Transactions on Information Systems, Vol. 23, No. 1, January 2005. 42 • J. Sander et al. Within one library, up to 80% of the unique tags have a frequency of 1, but these make up about 20% of the total number of copies of tags. Some of the single frequency tags represent genuine low frequency mRNAs. However, it is impossible to tell which of the single frequency tags are errors by only looking at a single library. Since the size of the set of possible tags is 410 ≈ 106 , and only about 3 × 105 of these tags have ever appeared in any library, a sequence error in a tag most likely represents a completely new tag that never appears in any other library.

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23, No. 1, January 2005. , bulk tissue, or cell line) can dominate the similarity between the tissues. We tried to weaken this kind of distortion by applying the test again to also determine the tags that have significantly different expression levels in bulk tissue versus cell line. Those tags were then subtracted from the set of potentially cancer relevant tags selected in the first step. To demonstrate the effect of our missing value imputation on the subspace selection method, we first applied the Wilcoxon test to the SAGE data after error removal and normalization, but without imputing missing values.

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ACM transactions on information systems (January) by Association for Computing Machinery

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